You can remove an app from a playlist that you have created by doing the following:
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While logged in to your Glitch account, click on your avatar which is located at the top-right corner of any page on glitch.com
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In the popup menu, click on your avatar. This will take you to your profile page.
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Click on the playlist that includes the app that you want to remove.
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In the playlist, look for the app that you want to remove.
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To the right of the app name, click the little down arrow to reveal the options menu.
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Click on Remove from Playlist.
Keep in mind that removing an app from a playlist does not delete the app itself.